Overview
Protein microarray analysis is a high-throughput technique in which large numbers of proteins, antibodies, or other capture molecules are immobilized in an ordered array on a solid surface, allowing the simultaneous study of many protein interactions, abundances, or activities in a single experiment. The platform enables miniaturized, parallel detection and is used to profile protein expression, map binding partners, screen for biomarkers, and characterize enzymatic and post-translational activity. Common formats include analytical or antibody arrays for measuring specific proteins in complex samples, functional arrays of purified proteins for studying interactions and modifications, and reverse-phase arrays for quantifying targets across many samples. As a component of proteomics, protein microarray analysis complements mass-spectrometry-based approaches such as quantitative proteomics using stable-isotope labelling, together building the protein inventories and interaction maps central to efforts like the human proteome project. The technology supports investigation of signalling and regulatory proteins, including the study of molecules such as optineurin in the control of chemokines and kinases, and the dissection of disease-related pathways, for example regulatory mechanisms in colorectal cancer. By revealing patterns of protein expression and interaction, it informs biomarker discovery, disease diagnosis, and drug development. Protein microarray analysis thus provides a scalable means of surveying the proteome, linking protein abundance and function to biological state and supporting both basic research and translational applications in disease characterization.
Research published in this journal
7 peer-reviewed articles, ranked by relevance. Each links to its DOI.
Human Proteome Project and Current Bioinformatics Status in Disease Diagnosis and Treatment
Proteomic and Genomic Techniques in Medical Research: Applications in Cancer, Diagnostics, and Personalized Medicine
Quantitative Proteomics Using 15N SILAC Mouse
Mechanisms Associated with Acquisition of Resistance to Butyrate-Induced Apoptosis in Colorectal Cancer Cells Using Gene Expression Analysis
Proteome and Proteomics: from Single Protein to Whole Body
GR-RNF43 Regulation in Colorectal Cancer
How this research is being cited
The 7 articles above have been cited 9 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.
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2021 · Nature Communications
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Marion Lenoir et al. · 2020 · Gut microbes
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2020 · Gut Microbes
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2020 · Research Square (Research Square)
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2018 · Journal of Proteomics and Genomics research
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2016 · PeerJ
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2016 · Journal of Proteomics and Genomics research
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2014 · The Journal of Neuroscience
A sample of recent works citing this journal's research on Protein Microarray Analysis, linking to each citing work.