Overview
Protein identification is the process of determining which proteins are present in a biological sample, a foundational task in proteomics that links the complex mixtures of cellular proteins to their underlying gene products and functions. Proteins are large biomolecules that provide structure, catalyze reactions, and transport and regulate molecules within cells, and identifying them reveals how the proteome varies with cell type, condition, and disease. The dominant analytical approach is mass spectrometry, in which proteins are typically digested into peptides whose mass and fragmentation spectra are measured and matched against sequence databases to infer identity. Strategies include shotgun and label-free proteomics for broad profiling, ion-current-based and targeted quantitative methods that compare protein abundance between states such as drug treatment, and affinity purification coupled with tandem mass spectrometry to characterize interacting proteins. These methods support the study of protein function, post-translational modification, and interaction networks, and they underpin large-scale efforts such as the Human Proteome Project and bioinformatic analyses that connect proteins to disease pathways. Accurate identification depends on sample preparation, instrument performance, reproducible workflows, and computational analysis of spectra. By revealing the identities and quantities of proteins in samples ranging from human tissue to parasites and model systems, protein identification advances biomarker discovery, mechanistic biology, and the diagnosis and treatment of disease.
Research published in this journal
10 peer-reviewed articles, ranked by relevance. Each links to its DOI.
Determination of the Proteomic Response to Lapatinib Treatment using a Comprehensive and Reproducible Ion-Current-Based Proteomics Strategy
Shotgun Label-Free Proteomic Analyses of the Oyster Parasite Perkinsus Marinus
Optimization and ZSPORE Analysis of Affinity Purification Coupled with Tandem Mass Spectrometry in Mammalian Cells
Human Proteome Project and Current Bioinformatics Status in Disease Diagnosis and Treatment
Proteome and Proteomics: from Single Protein to Whole Body
Effect of Nonionic Surfactants and HPMC F4M on the Development of Formulations of Neuro-EPO as a Neuroprotective Agent
Bioinformatic Analysis of Coronary Disease Associated SNPs and Genes to Identify Proteins Potentially Involved in the Pathogenesis of Atherosclerosis
The NH2-Terminal Ig Domains of Insect Projectin could serve as Elastic Elements
How this research is being cited
The 10 articles above have been cited 61 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.
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2026 · Biochemical Pharmacology
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2026 · Alcohol and Alcoholism
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2026 · BMC Bioinformatics
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Hami Hemati et al. · 2025 · Brain, behavior, and immunity
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2025 · Computational and Structural Biotechnology Journal
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2025 · Journal of Molecular Structure
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2025 · Brain Behavior and Immunity
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2025 · Journal of Molecular Structure
A sample of recent works citing this journal's research on Protein Identification, linking to each citing work.