Research Topic · Peer-Reviewed

Affinity Chromatography

Affinity chromatography is a separation technique that purifies a target molecule based on its specific, reversible binding to a partner immobilized on a solid support. A ligand, such as an antibody, antigen, enzyme substrate, metal ion, or other binding molecule, is attached to a stationary phase packed in a column…

Curated from this journal's research 📚 9 peer-reviewed articles cited Cited 16× across the literature 🔖 ISSN 2377-2549 🗓 Reviewed July 2026

Overview

Affinity chromatography is a separation technique that purifies a target molecule based on its specific, reversible binding to a partner immobilized on a solid support. A ligand, such as an antibody, antigen, enzyme substrate, metal ion, or other binding molecule, is attached to a stationary phase packed in a column. When a complex mixture passes through, only the target that recognizes the ligand is retained, while other components wash away; the bound target is then released, or eluted, by changing conditions such as pH, salt concentration, or by adding a competing molecule. Because it exploits highly selective biological recognition, affinity chromatography is widely used to purify proteins, antibodies, enzymes, and nucleic acids in research and biopharmaceutical production. Within the journal's coverage of New Developments in Chemistry, related work includes the isolation and characterization of antibodies and enzymes, areas in which selective binding and purification are central. This page gathers peer-reviewed, open-access research relevant to affinity chromatography and the broader separation science used to isolate biomolecules, offering a reference point for readers interested in protein purification, ligand-based separation, and the analytical and preparative methods that depend on molecular recognition.

Research published in this journal

9 peer-reviewed articles, ranked by relevance. Each links to its DOI.

2018

Enzymology in its Second Century

Brumm PhillipCorresponding author
C5-6 Technologies LLC, 5627 Old Oak Drive, Fitchburg, WI 53711, USA
Exact topic Enzymes doi:10.14302/issn.2690-4829.jen-18-2010

How this research is being cited

The 9 articles above have been cited 16 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.

A sample of recent works citing this journal's research on Affinity Chromatography, linking to each citing work.

Editorial oversight

Curated from peer-reviewed research published in New Developments in Chemistry (ISSN 2377-2549).

Journal editorial board
Annarita Del Gatto · Italy Bharat Gurale · United States Palani ELUMALAI · United Kingdom

This page summarises published research for orientation; it is not medical or professional advice.