Protein purification is the set of laboratory techniques used to isolate a specific protein from a complex biological mixture such as a cell lysate, tissue extract, or culture medium. By progressively separating the target protein from other proteins, nucleic acids, lipids, and small molecules, purification yields m…
Protein purification is the set of laboratory techniques used to isolate a specific protein from a complex biological mixture such as a cell lysate, tissue extract, or culture medium. By progressively separating the target protein from other proteins, nucleic acids, lipids, and small molecules, purification yields material pure enough to study a protein's structure, activity, and interactions or to use it in research, diagnostics, and pharmaceutical production. Common methods exploit differences in size, charge, hydrophobicity, and binding specificity, and include centrifugation, precipitation, and chromatographic approaches such as affinity, ion-exchange, and size-exclusion chromatography, often combined in multi-step schemes and monitored for yield and purity. Within this journal's scope on Biotechnology and Biomedical Science, related research includes the optimisation and analysis of affinity purification coupled with tandem mass spectrometry in mammalian cells, illustrating affinity-based isolation linked to protein identification, and the purification and characterisation of xanthine oxidase from sheep liver, demonstrating the isolation and study of an enzyme from tissue. This page gathers peer-reviewed, open-access research relevant to protein purification and the methods used to obtain and characterise individual proteins.
12 peer-reviewed articles, ranked by relevance. Each links to its DOI.
The 12 articles above have been cited 41 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.
A sample of recent works citing this journal's research on Protein Purification, linking to each citing work.