Chromatography

Overview

Chromatography is a family of analytical and preparative techniques used to separate the components of a mixture based on their differential partitioning between a stationary phase and a mobile phase. As analytes move through or across the stationary phase, differences in properties such as polarity, size, charge, or affinity cause them to migrate at different rates, achieving separation that enables identification and quantification. Major formats include thin-layer chromatography, widely applied to phytochemical profiling and the separation of pharmaceutical compounds, and high-performance liquid chromatography, frequently coupled with mass spectrometry to provide sensitive and specific detection. Liquid chromatography-tandem mass spectrometry is a mainstay for determining drug and metabolite concentrations in biological fluids, supporting bioequivalence and pharmacokinetic studies, and for analyzing complex matrices in food safety and natural-product chemistry. The technique is essential across biochemistry, pharmaceutical analysis, food science, and synthetic chemistry, where it is used to isolate and characterize secondary metabolites, verify the composition of formulations and supplements, and detect adulterants. Method development addresses the choice of stationary and mobile phases, detection strategy, and validation parameters such as sensitivity and selectivity. By resolving chemically similar species with high precision, chromatography underpins both routine quality control and advanced research into molecular structure, composition, and the behavior of compounds in biological and environmental systems.

Research published in this journal

12 peer-reviewed articles, ranked by relevance. Each links to its DOI.

How this research is being cited

The 12 articles above have been cited 85 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.

• Deciphering the detailed crystallographic structure of biologically synthesized nano-silver: Antioxidant potential, antimicrobial efficacy and anticancer properties against HepG2 cancer cell line with accentuation to apoptosis and ROS generation
  2026 · Journal of Molecular Structure
• Green-Synthesized titanium dioxide Nanoparticle–Modified glass ionomer cement: in vitro and in Silico assessment of Mechanical, Physical, and safety properties performance
  2026 · Scientific Reports
• Phytofabricated Silver Nanoparticles from Origanum majorana: Anti-Pathogenic Activity and Biocompatibility Evaluation
  2026 · Microbial Pathogenesis
• Antagonistic Bacterial Endophytes from Gingers and their Metabolite Mediated Interactions Against Pythium Soft Rot
  2026 · Current Microbiology
• Green Synthesis of Silver Nanoparticles Using Cynoglossum creticum Leaf Extract: Eco-Friendly Approach for Antibacterial, Antioxidant, and Sensing Applications
  2025 · Applied Biochemistry and Biotechnology
• Insights into synergistic antibacterial effects, antioxidant potential, and anticancer activities of green-synthesized silver nanoparticles from Odontosoria chinensis leaves
  2025 · Next Nanotechnology
• Antioxidant and ameliorative effects of selected Nigerian plants on hormonal imbalance associated with dysmenorrhea in albino rats
  2025 · Frontiers in Nutrition
• Metabolomics of Trichoderma spirale by Mass Spectrometry Data Dereplication
  2025 · Chemistry of Natural Compounds

A sample of recent works citing this journal's research on Chromatography, linking to each citing work.

This page summarises published research for orientation; it is not medical or professional advice.

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