Bacterial broth dilution is a laboratory method used to determine how well an antimicrobial agent inhibits or kills bacteria, and to quantify bacterial growth in liquid culture. In the technique, a bacterial sample is added to a nutrient broth containing serial, stepwise dilutions of an antimicrobial compound, and t…
Bacterial broth dilution is a laboratory method used to determine how well an antimicrobial agent inhibits or kills bacteria, and to quantify bacterial growth in liquid culture. In the technique, a bacterial sample is added to a nutrient broth containing serial, stepwise dilutions of an antimicrobial compound, and the cultures are incubated and examined for visible growth. The lowest concentration that prevents visible bacterial growth is recorded as the minimum inhibitory concentration (MIC), and further subculturing onto solid media can establish the minimum bactericidal concentration. Broth dilution can be performed in tubes (macrodilution) or in microplate wells (microdilution), and it is a standard approach for antimicrobial susceptibility testing. The method is important for guiding antibiotic selection, evaluating antibiotic resistance, and assessing the antibacterial efficacy of candidate compounds, natural extracts, disinfectants, and pharmaceutical formulations. As a quantitative susceptibility assay, it underpins much of the work in Advanced Pharmaceutical Science And Technology, where the discovery, formulation, and testing of antimicrobial agents and other therapeutics rely on reproducible measures of biological activity. This page gathers peer-reviewed, open-access research relevant to pharmaceutical science and the evaluation of bioactive compounds within the broad scope of Advanced Pharmaceutical Science And Technology.
1 peer-reviewed article, ranked by relevance. Each links to its DOI.
The 1 article above has been cited 98 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.
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